Western blot was performed using PIT1 Polyclonal Antibody (Product # PA5-84020) and a 32-34 kDa bands corresponding to PIT1/POU1F1 were observed across cell lines tested except HeLa and HEK-293. Whole cell extracts (40 µg lysate) of A-673 (Lane 1), HeLa (Lane 2) and HEK-293 (Lane 3) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX), 10 well. Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:20,000) using the iBright™ FL1500 Imaging System (Product # A44115). Chemiluminescent detection was performed using SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Product # 34580).
Immunohistochemical analysis of PIT1 in human pituitary gland using a PIT1 polyclonal antibody (Product # PA5-84020). The analysis shows strong nuclear positivity in anterior lobe.
Supplier Page from Thermo Fisher Scientific for PIT1 Antibody